These manga star protagonists who don't fit neatly into a male or female category. They might be genderless by nature, be biologically intersex, switch back and forth between genders, or not identify as male or female at all.
These manga feature LGBT themes that focus on gender and gender identity, including trans-related topics, rather than the sexuality side of the tag.
These couples start out hating each other/being on opposite sides of a conflict, only to fall in love by the end of the story.