Most overall disturbing anime.

[Doomoo]

What anime left you the most disturbed overall?

For me it's probably Shiki.
Runner ups would be:
2nd Higurashi no Naku Koro ni
3rd Elfen Lied
4th Another
5th GANTZ
6th Jigoku Shoujo
7th Shadow Star Narutaru

[Thrawn]

I think we all know what I'm gonna say.

That's right fuckers, Popee the Fucking Performer. When one starts watching this fuckballs amazing work of shit, their first thought might be "The fuck is this cock tits?" but a few episodes on, when you get to the more homoerotic stuff (Pre-Papi, shocking because although Papi is the fucking homoerotic sex god, he was there before, and his fucking sex aura still has influence), your next thought would be "Did he just eat a bullet from his ass?" and I shit you not, pun unintended fucker. But shit man, when Papi enters you, shit gets fucking weird as fuck. There's enough swaggering, implied sexual harassment, sexual harassment and fucking fuckfabulousness that you realize that you're now fucking milking from your now erect nipples.

But I'm getting ahead of myself. Getting to Poison, fucking great shit right there, your introduced mind to the fucking fabulousness of Papi, still your first time watching this shit; "Holy fucks of a cock bitch, my mind was fucking soiled like Poopy's throat. If it gets more homoerotic than this or any more- Holy fucks of a fuck, no fucking way can it top that!" but then... you reach the fabfuckulistic Sleep, the pinnacle of mind fuckery and homomilkism. When you first see it, it's like- Fuck, if I talk about what it's like, I'll get in trouble again. It'll fucking change your life and you'll never see milk bottles, clowns, fabulous sun deities or the fucking circus the same fucking way again.

Watching Sleep for the first time is a fucking life-changing event. It fucking warps your now sodomized mind in the ways of Papi the Fucking Fabulous Sun Sex God of Sodomizing Fertility and mentally scars your psyche for the rest of your fucking bitch ass life.

Fucking Sleep man. Can never mention that shit enough. The most disturbing shit ever and the fucking pinnacle and defining moment of this fucking so-bad-it's-fucking-good-as-fucks series, and not even that fucking creepy ass bastard Moon cradling his fly-attracting yellow shit can top that. And that, you sorrowful fucks, is one of the most horrifying, disturbing things my fucking eyes had the misfortune to ever look upon.

[AhnYS]

^Beat me to it.

[LizardLaw974]

Perfect Blue. Hands down

Elfen Lied and Serial Experiments Lain are on there too.

...and what Thrawn said.

[Bluntjoker]

Paprika all day long.

[Doomoo]

I just watched the episodes you linked to in your post Thrawn and I didn't find it that disturbing... I've been on the internet far too long... Weirdness and homoeroticism no longer affect me.

Originally Posted by Bluntjoker

Paprika all day long.

You found that to be disturbing?

[BrainBlow]

Eiken
That shit is just... just... what.

[Jensling]

If hentai counts, then Pidgeon Blood
If not, then... Perfect Blue or Paranoia Agent.

[PinkyIvan]

Elfen Lied... no, not really, except the blood and bone part it is a beautiful altruistic love story IMO. Since I've been reading Dylan Dog since I was 14 not much can disturb me... Ok Crossed and many many many hentai are still disturbing...
As for whose work can disturb normal people it is Mohiro Kitoh, the author whose work usually involves pedophilia, rape and general mental torturing of characters. He made Bokurano and Shadow Star Narutaru (and many other amazing works)

[Doomoo]

It's too bad they have not made an anime out of the manga Franken Fran. I'm sure that would probably top the list.